Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells
نویسندگان
چکیده
منابع مشابه
-dependent exocytosis from PC12 cells
Genes to Cells (2006) 11 , 659–672 Journal compilation © 2006 by the Molecular Biology Society of Japan/Blackwell Publishing Ltd. 659 DOI: 10.1111/j.1365-2443.2006.00970.x Blackwell Publishing Inc M lden, USA GTC enes to Cells 1365-2443 © Blackwell Publ shing Ltd ? 2006 1 6Original Artic e Ro of the ELKS/CAST family member in exocytosis E I ou et al. ELKS, a protein structurally related to the ...
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We have studied exocytosis in rat peritoneal mast cells by cell-attached patch amperometry. Step increases in capacitance were accompanied by typical amperometric spikes due to the release of 5-hydroxytryptamine (serotonin), indicating exocytosis of typical mast cell granules. We have measured the time course of fusion pore expansion, and correlated it with release from the granule matrix. The ...
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Introduction: Catecholamines are charged neurotransmitters found in the neuroendocrine cells called chromaffins. The release of catecholamine, through a narrow fusion pore of a chromaffin cell requires a charge compensation by other ions to maintain an osmotic balance of charges. The aim of this research is to show that the exocytotic catecholamine release is not associated with the cation flux...
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We have developed a cell-free system for regulated exocytosis in the PC12 neuroendocrine cell line. Secretory vesicles were preloaded with acridine orange in intact cells, and the cells were sonicated to produce flat, carrier-supported plasma membrane patches with attached vesicles. Exocytosis resulted in the release of acridine orange which was visible as a disappearance of labeled vesicles an...
متن کاملRelease of neurotransmitters from glia.
There is no question about the fact that astrocytes and other glial cells release neurotransmitters that activate receptors on neurons, glia and vascular cells, and that calcium is an important second messenger regulating the release. This occurs in cell culture, tissue slice and in vivo. Negative results from informative experiments designed to test the mechanism of calcium-dependent neurotran...
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ژورنال
عنوان ژورنال: Journal of Electroanalytical Chemistry
سال: 2016
ISSN: 1572-6657
DOI: 10.1016/j.jelechem.2016.10.025